- What happens to the image if you try to magnify it using 40x or 100x?
- What is the difference between high and low power objective?
- Why do you need more light at higher magnification?
- What happens to the field of view as magnification increases quizlet?
- What is the relationship between field of view and total magnification?
- What happens to depth of field as total magnification decreases?
- What happens to the field of view when you increase magnification?
- What 3 things change as you increase magnification?
- When using the high power objective you should not adjust the?
- What is a limitation of the light microscope explain why this is a limitation?
- Which objective lens will give you the greatest resolving power?
- Which objective lens should you always use first when viewing a new slide?

## What happens to the image if you try to magnify it using 40x or 100x?

5.

What happens to your image if you try to magnify it using 40x or 100x.

It could blow up your iage if you do not adjust the stage accordingly..

## What is the difference between high and low power objective?

When you change from low power to high power on a microscope, the high-power objective lens moves directly over the specimen, and the low-power objective lens rotates away from the specimen.

## Why do you need more light at higher magnification?

In general, the more light delivered to the objective lens, the greater the resolution. The size of the objective lens aperture (opening) decreases with increasing magnification, allowing less light to enter the objective. You will likely need to increase the light intensity at the higher magnifications.

## What happens to the field of view as magnification increases quizlet?

What is Field of View? … As magnification increases, the diameter of the field of view decreases. In other words, you can see less area of the specimen as you increase the magnification.

## What is the relationship between field of view and total magnification?

There is an inverse relationship between the total magnification and the diameter of the field of view – i.e., as magnifications increases the diameter of the field decreases in proportion, so the diameter of field of view at different magnification can be calculated mathematically, using the formula.

## What happens to depth of field as total magnification decreases?

What happens to depth of field when you increase magnification? Decrease magnification? The less overall thickness you can see, so the depth of field is less. Lower the magnification, the greater the thickness you can see, so the greater the depth of field.

## What happens to the field of view when you increase magnification?

FOV is inversely proportional to the magnification (as the magnification increases, the FOV decreases). Another way to understand this is to consider that when a specimen is magnified, the microscope is zooming in on it and, consequently, seeing less of it (but in greater detail).

## What 3 things change as you increase magnification?

The more you magnify an image, the thinner the light gets spread, and you reach the point where even with a very bright light, the image is too dark to see anything. We get around the problems of brightness and depth of field by using something besides light for the microscope.

## When using the high power objective you should not adjust the?

Never use the coarse adjustment when using high power. Doing so could break the slide or the microscope! 17. Watching from the side, switch to the high-power objective lens.

## What is a limitation of the light microscope explain why this is a limitation?

The main limitation of light microscopes are, that they have a lower resolution and cannot see beyond 200nm. And it cannot see smaller organelles like ribosomes.

## Which objective lens will give you the greatest resolving power?

Oil Immersion ObjectiveIn a compound light microscope, the objective lens that will give you the greatest resolving power is the Oil Immersion Objective (OIO).

## Which objective lens should you always use first when viewing a new slide?

ALWAYS use both hands when picking the microscope up and moving it from one place to another. 3. When focusing on a slide, ALWAYS start with either the 4X or 10X objective. Once you have the object in focus, then switch to the next higher power objective.