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The rats were dark adapted for the electroretinogram ERG recording overnight. Immunohistochemistry of Retinal Flatmounts After 3 and 14 Days Three and 14 days after the intraocular injection of PBS or SB, eyes were prepared for flatmounts as previously described Casola et al. Acta , — Therefore, a low pass filter Hz was necessary. Preparation of human formalin-fixed brain slices for electron microscopic investigations.
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The a- and b-wave amplitudes of the retina were investigated via ERG. The intraocular injection of SB is a new model for a glaucoma like degeneration. At day 3, a slight damage of the optic nerve was noted, but the retina was not yet affected. European glaucoma society terminology and guidelines for glaucoma, 4th edition—chapter 2. Look, All i wanted was some opinions on if I should or not. A We performed immunohistochemical evaluations of amacrine cells calretinin, green 14 and 21 days after SB injection. Scotopic flash series were recorded with light flash intensity at 0.
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SB is a protein which regulates the intracellular calcium level Donaldson et al. Because they are crap. Retinal ganglion cell loss is accompanied by antibody depositions and increased levels of microglia after immunization with retinal antigens. Also, the optic nerve neurofilament structure was damaged from day 3 on.
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